Glycine and glycine receptor immunoreactivity in brain and spinal cord

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Glycine and glycine receptor immunoreactivity in brain and spinal cord.

To study the distribution of glycine immunoreactive neurons in the spinal cord and brain, antisera were raised against glycine conjugated to protein carriers. High-titer rabbit glycine antiserum was purified by affinity chromatography. Testing against other amino acids and peptides with immuno dot blots and ELISA assays showed little apparent cross-reaction with glutamate, aspartate, glutamine,...

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Glycine-immunoreactivity in the auditory brain stem of the chick.

Glycine-immunoreactivity (Gly-I) was studied in the auditory brain stem of chick using a polyclonal antiserum to glycine conjugated to bovine serum albumin (Wenthold et al., 1987). During embryonic development, little Gly-I is present in nucleus magnocellularis (NM), nucleus laminaris (NL), or nucleus angularis (NA). In posthatch chicks, a few Gly-I terminals are found on nerve cell bodies in N...

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Cloning and expression of a spinal cord- and brain-specific glycine transporter with novel structural features.

A novel glycine transporter (GLYT2) was cloned from a rat brain cDNA library. GLYT2 is about 48 and 50% homologous to the previously cloned mouse glycine transporter (GLYT1) and rat proline transporter (PROT), respectively. GLYT2 differs from GLYT1 in molecular structure, tissue specificity, and pharmacological properties. The cDNA of GLYT2 encodes for 799 amino acid residues with an extended a...

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Specific glycine--accumulating synaptosomes in the spinal cord of rats.

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ژورنال

عنوان ژورنال: The Journal of Neuroscience

سال: 1988

ISSN: 0270-6474,1529-2401

DOI: 10.1523/jneurosci.08-02-00472.1988